PAD4 is a pool of three individual synthetic siRNA duplexes designed to knock-down human PAD4 mRNA expression. Each siRNA is 19-25 bases in length.
Catalog No. P312-911D
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PAD4 is a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions (1). PAD4 is essential for antibacterial innate immunity mediated by neutrophil extracellular traps (NETs) (2). PAD4 play a role in granulocyte and macrophage development leading to inflammation and immune response. PAD4 also mediates the gene expression by regulating arginine methylation and citrullination in histones(3).
PADI4; PAD; PDI4; PDI5
1. Nakashima, K. et.al: Molecular characterization of peptidylarginine deiminase in HL-60 cells induced by retinoic acid and 1-alpha,25-dihydroxyvitamin D(3). J. Biol. Chem. 274: 27786-27792, 1999.
2. Li, P.et.al: PAD4 is essential for antibacterial innate immunity mediated by neutrophil extracellular traps. J. Exp. Med. 207: 1853-1862, 2010.
3. Wang, Y. et.al: Human PAD4 regulates histone arginine methylation levels via demethylimination. Science 306: 279-283, 2004.
PAD4 siRNAs are designed to specifically knock-down human PAD4 expression.
Each siRNA duplex is supplied as 2.5nmol per tube (for example 6 x 2.5nmol = 3 x 5nmol per duplex). Briefly centrifuge one tube per duplex (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend each 2.5nmol tube in 50 ul of DEPC-treated water (included in kit), which results in a 1x stock solution (50 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Storage and Stability:
The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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