The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. p85α modulates the interaction between PI3K and platelet-derived growth factor receptor (1). Furthermore, estrogen receptor isoform ER-alpha binds in a ligand-dependent manner to the p85-alpha regulatory subunit of PI3K. Stimulation with estrogen increases ER-alpha-associated PI3K activity, leading to the activation of protein kinase B/AKT and endothelial nitric oxide synthase (eNOS) (2).
PIK3R1, GRB1, p85-ALPHA
1. Skolnik, E. Y. Et al: Cloning of PI3-kinase associated p85 utilizing a novel method for expression/cloning of target proteins for receptor tyrosine kinases. Cell 65: 83-90, 1991.
2. Simoncini, T. et al: Interaction of oestrogen receptor with the regulatory subunit of phosphatidylinositol-3-OH kinase. Nature 407: 538-541, 2000.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Kong Kathleen et al., The human adenovirus E4-ORF1 protein subverts discs large 1 to mediate membrane recruitment and dysregulation of phosphatidylinositol 3-kinase PLoS Pathogens May 2014 10.1371/journal.ppat.1004102
M. Singel Stina et al., KIF14 Promotes AKT Phosphorylation and Contributes to Chemoresistance in Triple-Negative Breast Cancer Neoplasia March 2014 10.1016/j.neo.2014.03.008
ML Lin et al., Suppression of PI3K/Akt signaling by synthetic bichalcone analog TSWU-CD4 induces ER stress-and Bax/Bak-mediated apoptosis of cancer cells Apoptosis : an International Journal on Programmed Cell Death November 2014 10.1007/s10495-014-1031-y
L Maasa Nancy et al., Generation and characterization of an analog-sensitive PERK allele Cancer Biology & Therapy May 2014 10.4161/cbt.29274
Furukawa S et al., PI3Kδ Inhibition Augments the Efficacy of Rapamycin in Suppressing Proliferation of Epstein?Barr Virus (EBV)+ B Cell Lymphomas American Journal of Transplantation July 2013 10.1111/ajt.12328
Vidugiriene Jolanta et al., Evaluating the Utility of a Bioluminescent ADP-Detecting Assay for Lipid Kinases ASSAY and Drug Development Technologies January 2010 10.1089/adt.2009.0223
AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Lipid Kinases, Metabolic Disorder, Neurobiology, NfkB Pathway, WNT Signaling