GFP or green fluorescent protein generates a highly visible efficiently emitting internal fluorophore and has been extensively used as a marker of gene expression and protein targeting in intact cells and organisms (1). This GFP was originally used in the Neisseria gonorrhoeae expression plasmid pCmGFP (2) and belongs to the cl08319 superfamily (3).
1. Tsien RY. The green fluorescent protein. Annu Rev Biochem. 67:509-44, 1998.
2. Srikhanta,Y.N. et al: Phasevarions mediate random switching of gene expression in pathogenic Neisseria. PLoS Pathog. 5 (4), E1000400, 2009.
3. Marchler-Bauer A et al., "CDD: a Conserved Domain Database for the functional annotation of proteins.", Nucleic Acids Res.39(D)225-9, 2011.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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