Recombinant full-length human BAD was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. B03-30G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
B03-30G-20 | 20 ug | $215 | |
B03-30G-50 | 50 ug | $435 | |
B03-30G-BULK | BULK | Contact Us |
Overview:
BAD is a member of the BCL-2 family of proteins that are known to be regulators of programmed cell death. BAD is a pro-apoptotic protein that forms a heterodimer complex with BCL-xL and BCL-2 which reverses the prosurvival activity of these proteins (1). The proapoptotic activity of BAD is regulated through its phosphorylation and this inhibits the pro-apoptosis function of BAD. Protein kinases such as AKT, RAF and RSK1 can phosphorylate BAD and RSK1-induced phosphorylation of BAD at ser112 suppresses BAD-mediated apoptosis in neurons. BAD inhibits G(1) to S phase transition in MCF7 breast cancer cells and overexpression of BAD inhibits cell growth as well as cyclin D1 expression (2).
Gene Aliases:
BBC2, BCL2L8
Genbank Number:
References:
1. Yang E, et al: Bad, a heterodimeric partner for Bcl-X(L) and Bcl-2, displaces Bax and promotes cell death. Cell 80: 285-291, 1995.
2. Fernando R, et al: Breast cancer cell proliferation is inhibited by BAD: regulation of cyclin D1. J Biol Chem. 2007 Sep 28;282(39):28864-73.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~47 kDa
Apoptosis/Autophagy, Cancer, Cellular Stress, Neurobiology
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