Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
S54-63R-25	25 ug	$110
S54-63R-100	100 ug	$395
S54-63R-BULK	BULK	Contact Us

Overview:

STAT3 is a member of the signal transducers and activators of transcription (STAT) family of proteins that carry out a dual function: signal transduction and activation of transcription. STAT3 is widely expressed and becomes activated through phosphorylation on tyrosine as a DNA binding protein in response to a variety of stimuli such as EGF, IL-6, PDGF, IL-2 and G-CSF (1). This phosphoprotein forms homodimers as well as heterodimers with other members of the STAT family and translocate to the nucleus in order to modulate the transcription of various genes (2).

References:

1. Zhong, Z. et al: Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6. Science. 1994 Apr 1;264(5155):95-8.

2. Tian, S. S. et al: Rapid activation of the STAT3 transcription factor by granulocyte colony-stimulating factor. Blood. 1994 Sep 15;84(6):1760

Specificity:

Recognizes the STAT3 protein

Cross Reactivity:

Western blot of human STAT3

STAT3 from other species may also be detectable.

Host:

Rabbit

Immunogen:

Protein Code: P40763

Purification:

Affinity chromatography

Stability:

Store at 4°C (add 0.1% NaN₃) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Representative western blot with Anti-STAT3 (1:250) using 20 ng of GST tagged human recombinant STAT1β, STAT2, STAT3, STAT4, STAT5, and STAT6.

Product Datasheets

H257-2

Yamamoto Masahiro et al., A single polymorphic amino acid on Toxoplasma gondii kinase ROP16 determines the direct and strain-specific activation of Stat3 Journal of Experimental Medicine November 2009 10.1084/jem.20091703

K. Urlam Murali et al., Development of new N-arylbenzamides as STAT3 dimerization inhibitors Medicinal Chemistry Communication April 2013 10.1039/C3MD20323A

Kim Daejin et al., A specific STAT3-binding peptide exerts anti-proliferative effects and antitumor activity by inhibiting STAT3 phosphorylation and signaling Cancer Research February 2014 10.1158/0008-5472.CAN-13-2187

Zhang Xiaolei et al., A novel inhibitor of STAT3 homodimerization selectively suppresses STAT3 activity and malignant transformation Cancer Research January 2013 10.1158/0008-5472.CAN-12-3175

Li Zhi et al., Two Rare Disease-Associated Tyk2 Variants Are Catalytically Impaired but Signaling Competent Journal of Immunology March 2013 10.4049/jimmunol.1203118

Jin Jeon Young et al., Role of NEK6 in Tumor Promoter-induced Transformation in JB6 C141 Mouse Skin Epidermal Cells Journal of Biological Chemistry September 2010 10.1074/jbc.M110.137190

CC Arpin et al., Applying Small Molecule Signal Transducer and Activator of Transcription-3 (STAT3) Protein Inhibitors as Pancreatic Cancer Therapeutics Molecular Cancer Therapeutics May 2016 10.1158/1535-7163.MCT-15-0003

Mazouchia Amir et al., On the performance of bioanalytical fluorescence correlation spectroscopy measurements in a multiparameter photon-counting microscope Analytica Chimica Acta February 2011 10.1016/j.aca.2011.01.002

A. Cumaraswamy Abbarna et al., Nanomolar-Potency Small Molecule Inhibitor of STAT5 Protein ACS Medical Chemistry Letters September 2014 10.1021/ml500165r

Giri Shailendra et al., Preclinical Therapeutic Potential of a Nitrosylating Agent in the Treatment of Ovarian Cancer PLoS One June 2014 10.1371/journal.pone.0097897

Kinoshita Takayoshi et al., Knowledge-Based Identification of the ERK2/STAT3 Signal Pathway as a Therapeutic Target for Type 2 Diabetes and Drug Discovery Chemical Biology & Drug Design July 2011 10.1111/j.1747-0285.2011.01151.x

Zeidan N et al., Magnetic bead-based electrochemical detection of interaction between epigallocatechin-3-gallate and STAT proteins Analytical Methods March 2015 10.1039/c5ay00169b

Salvatore Badali Daniel et al., Development of STAT3 as an accessible target for fluorescence-based inhibition assays Journal of Undergraduate Life Sciences May 2010

Danielle Croucher et al., Targeting the STAT3 signaling pathway in cancer: role of synthetic and natural inhibitors. Thesis; University of Toronto July 2013 10.1016/j.bbcan.2013.12.005

Anti-STAT3

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