Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
P02-63R-100	100 ug	$347
P02-63R-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

P02-63R-100

100 ug

$347

P02-63R-BULK

BULK

Overview:

PAK1 is a member of the p21-activated kinases (PAKs) which have been implicated in the regulation of cell morphology, motility and transformation. These serine/threonine kinases are activated by and are effectors of small GTPases, CDC42 and RAC. PAK1 belongs to the Group I PAKs which also includes PAK2 and PAK3 (1). PAK1 is a key regulator of the actin cytoskeleton, adhesion and cell motility. Inactive dimeric PAK1 is mainly cytosolic and interaction with the activators Cdc42-GTP and Rac1-GTP stimulates the kinase at the sites of cellular protrusions forming adhesions to the extracellular matrix (2).

References:

1. Jaffer, Z M. et al: p21-activated kinases: three more join the Pak. Int J Biochem Cell Biol. 2002 Jul;34(7):713-7.

2. Parrini, M C. et al: Spatiotemporal regulation of the Pak1 kinase. Biochem Soc Trans. 2005 Aug;33(Pt 4):646-8.

Specificity:

Recognizes the PAK1 protein

Cross Reactivity:

Western blot PAK1 from human and mouse cells

PAK1 from other species may also be detectable

Host:

Rabbit

Immunogen:

Synthetic peptide corresponding to amino acids 210-214 of human PAK1

Purification:

Affinity chromatography

Stability:

Store at 4°C (add 0.1% NaN₃) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Representative western blot with Anti-PAK1 (1:1000) using 3 μg, 10 μg, and 30 μg of Jurkat cel lysate.