Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
S56-63BR-100	100 ug	$347
S56-63BR-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

S56-63BR-100

100 ug

$347

S56-63BR-BULK

BULK

Overview:

STAT5 (signal transducers and activators of transcription 5) is a member of the STAT family. It is phosphorylated by receptor associated protein kinases in response to cytokines and growth factors (1). The differentiation of T helper cells is regulated by members of the STAT family. In BCR-ABL-positive cells, STAT5 is constitutively activated by tyrosine phosphorylation. STAT5 activation results in upregulation of Bcl-XL and increased resistance to induction of apoptosis. STAT5 is involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6 betahydroxylase (2).

References:

1. Wang, D. et al: Naturally occurring dominant negative variants of Stat5. Molec. Cell Biol. 16: 6141-6148, 1996.

2. A Subramanian et al: STAT 5 and NF-Y are involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6beta-hydroxylase. Nucleic Acids Res. 1998; 26(9): 2173–2178.

Specificity:

Recognizes the STAT5 protein

Cross Reactivity:

Western blot STAT5 from human cells

STAT5 from other species may also be detectable.

Host :

Rabbit

Immunogen:

Synthetic peptide corresponding to amino acids 692-696 of human STAT5 (protein accession number P42229)

Purification:

Affinity Chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Representative western blot with Anti-STAT5 (1:1000) using 5 ng of human recombinant STAT1β, STAT2, STAT3, STAT4, STAT5 and STAT6.