Recombinant full-length human CDK2 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Catalog No. C29-10G
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| C29-10G-05 | 5 ug | $226 | |
| C29-10G-10 | 10 ug | $325 | |
| C29-10G-BULK | BULK | Contact Us |
Overview:
CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B) (1). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition (2).
Gene Aliases:
CDK2: p33(CDK2) CyclinA2: CCN1; CCNA
References:
1. Levkau, B. et al: Cleavage of p21(Cip1/Waf1) and p27(Kip1) mediates apoptosis in endothelial cells through activation of Cdk2: role of a caspase cascade. Molec. Cell 1: 553-563, 1998.
2. Huang, H. et al: CDK2-dependent phosphorylation of FOXO1 as an apoptotic response to DNA damage. Science 314: 294-297, 2006.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
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Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
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Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~58 kDa and CyclinA2 ~78 kDa
M Kanke et al., Cohesin acetylation and Wapl-Pds5 oppositely regulate translocation of cohesin along DNA. EMBO Journal November 2016 10.15252/embj.201695756
Sankaranarayanan R et al., The Flavonoid Metabolite 2,4,6-Trihydroxybenzoic Acid Is a CDK Inhibitor and an Anti-Proliferative Agent: A Potential Role in Cancer Prevention. Cancers March 2019 10.3390/cancers11030427
A A et al., Regulation of C/EBPbeta1 by Ras in mammary epithelial cells and the role of C/EBPbeta1 in oncogene-induced senescence Oncogene November 2010 10.1038/onc.2010.336
Liu Shu et al., Structural and functional analysis of cyclin D1 reveals p27 and substrate inhibitor binding requirements ACS Chemical Biology August 2012 10.1021/cb1001262
de Pedroa Nuria et al., Protective effects of isolecanoric acid on neurodegenerative in vitro models Neuropharmacology October 2015 10.1016/j.neuropharm.2015.09.029
Lucchetti C et al., The Prolyl Isomerase Pin1 Acts Synergistically with CDK2 to Regulate the Basal Activity of Estrogen Receptor ? in Breast Cancer PLoS One February 2013 10.1371/journal.pone.0055355
Dachineni Rakesh et al., Salicylic acid metabolites and derivatives inhibit CDK activity: Novel insights into aspirin's chemopreventive effects against colorectal cancer Int J Oncol. October 2017 10.3892/ijo.2017.4167
McInnes C et al., Cyclin based inhibitors of CDK2 and CDK4 Patent US9376465 B2 June 2016
Bhandaria Deepali et al., Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration?proliferation dichotomy PNAS July 2015 10.1073/pnas.1514157112
et al., Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production Cell reports January 2020
et al., Discovery of novel cyclin-dependent kinase (CDK) and histone deacetylase (HDAC) dual inhibitors with potent in vitro and in vivo anticancer activity. European journal of medicinal chemistry October 2020
et al., Phosphorylation of mixed lineage kinase MLK3 by cyclin-dependent kinases CDK1 and CDK2 controls ovarian cancer cell division The Journal of Biological Chemistry July 2022
et al., Mutations at multiple CDK phosphorylation consensus sites on Cdt2 increase the affinity of CRL4 Cdt2 for PCNA and its ubiquitination activity in S phase. Genes to cells : devoted to molecular & cellular mechanisms September 2018
Cancer, Cell Cycle, Ser/Thr Kinases
BCR Protein, B01-35G
Tau-381 Protein, T04-54N
CDK6/CyclinD3, Unactive, C35-14H
CyclinO Protein, C85-30G
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