CK1ε is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair. CK1ε is a regulator of Yes-associated protein (YAP) transcription coactivator which is a key regulator of organ size and a candidate human oncogene. CK1ε is activated by CCK2R and this then phosphorylates PKD2 at Ser244. Phosphorylation of PKD2 leads to its nuclear accumulation and efficient phosphorylation of nuclear PKD2 substrates in human gastric cancer cells (1). CKIε can phosphorylate topoisomerase (topo) IIalpha at serine-1106 and this regulates the enzyme activity and sensitivity to topo II-targeted drugs (2).
CSNK1E, HCKIE, MGC10398, CK1E
1. von Blume J. et al: Phosphorylation at Ser244 by CK1 determines nuclear localization and substrate targeting of PKD2. EMBO J. 2007 Nov 14;26(22):4619-33.
2. Grozav, A G. et al: Casein kinase I delta/epsilon phosphorylates topoisomerase IIalpha at serine-1106 and modulates DNA cleavage activity. Nucleic Acids Res. 2009 Feb;37(2):382-92.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
R Chia et al., Phosphorylation of LRRK2 by casein kinase 1α regulates trans-Golgi clustering via differential interaction with ARHGEF7. Nature Communications December 2014 10.1038/ncomms6827
Zheng Y et al., Regulation of folate and methionine metabolism by multisite phosphorylation of human methylenetetrahydrofolate reductase scientific reports March 2019 10.1038/s41598-019-40950-7
Lee KW et al., Orobol, an Enzyme-Convertible Product of Genistein, exerts Anti-Obesity Effects by Targeting Casein Kinase 1 Epsilon scientific reports June 2019 10.1038/s41598-019-43950-9
A. Basta Holly et al., Encephalomyocarditis Virus Leader Is Phosphorylated by CK2 and Syk as a Requirement for Subsequent Phosphorylation of Cellular Nucleoporins Journal of Virology December 2013 10.1128/JVI.03150-13
Mamais Adamantios et al., Arsenite Stress Down-regulates Phosphorylation and 14-3-3 Binding of Leucine-rich Repeat Kinase 2 (LRRK2), Promoting Self-association and Cellular Redistribution Journal of Biological Chemistry June 2014 10.1074/jbc.M113.528463
Neise Denise et al., Evidence for a differential modulation of p53-phosphorylating kinases by the cyclin-dependent kinase inhibitor p21WAFI/C1P1 Cell Cycle September 2010 10.4161/cc.9.17.12799
Cancer, Inflammation, Neurobiology, NfkB Pathway, Ser/Thr Kinases, WNT Signaling