Recombinant full-length human SMAD3 was expressed in E. coli cells using an N-terminal GST tag.
Catalog No. S12-30G
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SMAD3 is a direct mediator of transcriptional activation by the TGFβ receptor. The activity of SMAD3 is regulated by the TGFβ receptors, and SMAD3 is phosphorylated and associated with the ligand-bound receptor complex. TGFβ stimulation leads to phosphorylation and activation of SMAD3, which form a complex with SMAD4 that accumulate in the nucleus and regulate transcription of target genes such as CDK inhibitor (1). SMAD3 containing a C-terminal truncation acts as a dominant-negative inhibitor of the normal TGFβ response. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2 (2).
MADH3, JV15-2, HSPC193, HsT17436, MGC60396, DKFZP586N0721, DKFZp686J10186
1. Inman, G. J. et al: Nucleocytoplasmic shuttling of Smads 2, 3, and 4 permits sensing of TGF-beta receptor activity. Molec. Cell 10: 283-294, 2002.
2. Matsuura, I. et al: Cyclin-dependent kinases regulate the antiproliferative function of Smads. Nature 430: 226-231, 2004.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Jiang Yanjun et al., Phosphatase PRL-3 Is a Direct Regulatory Target of TGFβ in Colon Cancer Metastasis Cancer Research January 2011 10.1158/0008-5472.CAN-10-1487
Li Zuguo et al., Response Gene to Complement 32 Is Essential for Fibroblast Activation in Renal Fibrosis Journal of Biological Chemistry December 2011 10.1074/jbc.M111.259184
et al., TGF?/SMAD signalling modulates MLL and MLL-AF4 mediated 5-lipoxygenase promoter activation. Prostaglandins & other lipid mediators July 2018
AKT/PKB Pathway, Angiogenesis, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Inflammation, JAK/STAT Pathway, JNK/SAPK Pathway, NfkB Pathway, WNT Signaling