PLK1 is a member of the Polo-Like Kinase family that localize to centrosomes or spindle pole bodies and undergo dramatic subcellular relocation during the cell cycle. Deregulated activities of PLKs often result in abnormalities in centrosome duplication, maturation, and/or microtubule dynamics (1). PLKs also regulate the function of the Golgi complex. Deregulated expression of human PLK1 is strongly correlated with the development of many types of malignancies, and ectopic expression of PLK1 dominant negative protein leads to rapid cell death (2).
1. Nigg, EA. et al: Dynamic changes in nuclear architecture during mitosis: on the role of protein phosphorylation in spindle assembly and chromosome segregation. Exp Cell Res. 1996 Dec 15;229
2. Dai, W. et al: Polo-like kinases and the microtubule organization center: targets for cancer therapies. Prog Cell Cycle Res. 2003;5:327-34..
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
De Blasio C et al., PLK1 targets NOTCH1 during DNA damage and mitotic progression The journal of Biological Chemistry October 2019 10.1074/jbc.RA119.009881 Free full text
K Zhu et al., Phospho-Pon Binding-Mediated Fine-Tuning of Plk1 Activity. Structure May 2016 10.1016/j.str.2016.04.012
C Victoria et al., Stathmin and microtubules regulate mitotic entry in HeLa cells by controlling activation of both Aurora kinase A and Plk1 Molecular Biology of the Cell October 2013 10.1091/mbc.E13-02-0108
HJ Chung et al., Phosphorylation of Astrin Regulates Its Kinetochore Function. Journal of Biological Chemistry August 2016 10.1074/jbc.M115.712745
Benadaa Jan et al., Polo-like kinase 1 inhibits DNA damage response during mitosis Cell Cycle January 2015 10.4161/15384101.2014.977067
Kassardjian Ari et al., The Transcription Factor YY1 Is a Novel Substrate for Aurora B Kinase at G2/M Transition of the Cell Cycle PLoS One November 2012 10.1371/journal.pone.0050645
Rizkallah Raed et al., The Transcription Factor YY1 Is a Substrate for Polo-Like Kinase 1 at the G2/M Transition of the Cell Cycle PLoS One January 2011 10.1371/journal.pone.0015928
Cancer, Cell Cycle, Ser/Thr Kinases