PDE3B is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP and cGMP. PDE3B has high affinity for cAMP and is inhibited by cGMP as well as specific inhibitors such as cilostamide and milrinone. PDE3B is phosphorylated and activated in response to insulin and agents that increase cAMP. Leptin can increase the activity of PDE3B in the hypothalamus leading to a decrease in cAMP concentration (1). PDE3B-knockout mice exhibited multiple alterations in regulation of lipolysis, lipogenesis, and insulin secretion, as well as signs of peripheral insulin resistance (2).
1. Zhao A Z, et al: A phosphatidylinositol 3-kinase-phosphodiesterase 3B-cyclic AMP pathway in hypothalamic action of leptin on feeding. Nature Neurosci. 5: 727-728, 2002.
2. Choi Y H, et al: Alterations in regulation of energy homeostasis in cyclic nucleotide phosphodiesterase 3B-null mice. J. Clin. Invest. 116: 3240-3251, 2006.
Sample Phosphodiesterase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Huang Lei et al., Phosphoinositide 3-Kinase Gamma Contributes to Neuroinflammation in a Rat Model of Surgical Brain Injury Journal of Neuroscience July 2015 10.1523/JNEUROSCI.0546-15.2015
Hiramotoa Kenichi et al., Role of phosphodiesterase 2 in growth and invasion of human malignant melanoma cells Cellular Signaling March 2014 10.1016/j.cellsig.2014.03.031
B Bolanda Brandon et al., Monomethylated-adenines potentiate glucose-induced insulin production and secretion via inhibition of phosphodiesterase activity in rat pancreatic islets Islets July 2015 10.1080/19382014.2015.1073435
Cardiovascular Disease, Inflammation, Phosphodiesterases