PDE4D is a member of the phosphodiesterase (PDE) family that is cAMP-specific and has homology to the 'dunce' gene of Drosophila. PDE4D is expressed in both cytosolic and particulate fractions in the cell and Rolipram, a specific PDE4 inhibitor, inhibits and significantly lowers the IC50 for the cytosolic forms of the enzyme. TNFα upregulates the basal expression of PDE4D in HUVEC. PDE4D, which is not detected in untreated cells, accumulates beginning 4 hours after TNFα treatment and increased by 24 hours (1). Mice deficient in PDE4D exhibit delayed growth as well as reduced viability and female fertility (2).
DPDE3; STRK1; HSPDE4D; PDE4DN2
1. Miro X, et al: Phosphodiesterases 4D and 7A splice variants in the response of HUVEC cells to TNF-alpha. Biochem. Biophys. Res. Commun. 274: 415-421, 2000.
2. Jin S-L C, et al: Impaired growth and fertility of cAMP-specific phosphodiesterase PDE4D-deficient mice. Proc. Nat. Acad. Sci. 96: 11998-12003, 1999.
Sample Phosphodiesterase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Jun Park Sung et al., Resveratrol Ameliorates Aging-Related Metabolic Phenotypes by Inhibiting cAMP Phosphodiesterases Cell February 2012 10.1016/j.cell.2012.01.017
S Valente et al., 1,4-Dihydropyridines Active on the SIRT1/AMPK Pathway Ameliorate Skin Repair and Mitochondrial Function and Exhibit Inhibition of Proliferation in Cancer Cells. Journal of Medicinal Chemistry February 2016 10.1021/acs.jmedchem.5b01117
Hiramotoa Kenichi et al., Role of phosphodiesterase 2 in growth and invasion of human malignant melanoma cells Cellular Signaling March 2014 10.1016/j.cellsig.2014.03.031
Cardiovascular Disease, Inflammation, Phosphodiesterases