Specificity:

Recognizes native and denatured forms of RFP protein.

Cross Reactivity:

Western blot RFP and its variants tag-RFP, DeRed, mCherry and mOrange, etc

Other RFP variants may also be detectable.

Host / Isotype / Clone#:

Mouse, IgG1, RF5R

Immunogen:

RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Representative western blot with Anti-RFP (1:1000) using 5 μl, 10 μl and 20 μl of 293 cell lysate transfected with a RFP protein.