Human ACE2 Stable Cell line-CHO
CHO cell line stably overexpressing native human angiotensin-converting enzyme 2 (ACE2) extracellular domain.
Catalog No. A51C2-71C
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CHO cell line stably overexpressing native human angiotensin-converting enzyme 2 (ACE2) extracellular domain.
Catalog No. A51C2-71C
Overview:
The angiotensin converting enzyme 2 (ACE2) belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases. ACE2 cleaves the angiotensin I and angiotensin II to counterbalancing ACE activity in the regulation of renin angiotensin system (RAS). ACE2 also has the role in regulation of cardiovascular, renal and reproductive systems physiologically (1). In addition, ACE2 is a functional receptor for the spike glycoprotein (S) of SARS-CoV, HCoV-NL63, and SARS-CoV-2 (2), which causes the pandemic of the respiratory diseases (COVID-19) (3).
Genbank Number:
References:
1. Imai Y, et al: Angiotensin-converting enzyme 2 (ACE2) in disease pathogenesis. Circ J. 2007, 74(3):405-10.
2. Lan J, et al: Crystal structure of the 2019-nCov spike receptor-binding domain bound with the ACE2 receptor. bioRxiv. doi: https://doi.org/10.1101/2020.02.19.956235
3. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.
Activity:
FACS assay showed 2019-nCoV Spike protein S1 can bind to ACE2 overexpressing cells. ACE2 overexpressing cells were stained with 2019-nCoV Spike protein S1, followed by anti-spike protein antibody (C19S1-61H) and fluorescence-conjugated secondary antibody.
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Subculture Routine:
Subculture the cells every two to three days
Stability:
1yr stored below –130oC from date of shipment
Storage and Shipping:
For optimal storage, store product in liquid nitrogen vapor until ready to use. Product shipped on dry ice.
Culture Medium:
DMEM + 5% FBS for adherent culture / HyClone HyCell for suspension culture.
Viability:
Cells recover as healthy logarithmically growing cells within 3 to 4 days post thawing. Viability measured to be ≥90%.
There are no related publications available for this product.
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