Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
S36-463R-100	100 ug	$595
S36-463R-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

S36-463R-100

100 ug

$595

S36-463R-BULK

BULK

Overview:

SIRT2 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT2 being a member of class I. Inhibition of SIRT2 can lead to neuroprotection in cellular and invertebrate models of Huntington's disease (1). Huntington's disease is characterized by increased sterol synthesis in neuronal cells and this process is reversed by SIRT2 inhibition. SIRT2 can deacetylate lys40 of alpha-tubulin both in vitro and in vivo (2). Knockdown of SIRT2 via small interfering RNA results in tubulin hyperacetylation.

References:

1. Luthi-Carter, R. Et al: SIRT2 inhibition achieves neuroprotection by decreasing sterol biosynthesis. Proc Natl Acad Sci U S A. 2010 Apr 27;107(17):7927-32.

2. North, B. J. et al: The human Sir2 ortholog, SIRT2, is an NAD(+)-dependent tubulin deacetylase. Molec. Cell 11: 437-444, 2003.

Specificity:

Recognizes the SIRT2 protein

Cross Reactivity:

Human, Mouse and Rat

Host / Isotype / Clone#:

Rabbit, IgG

Immunogen:

SIRT2 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of the human SIRT2

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Western blot analysis of SIRT2 in Mouse brain lysate with SIRT2 antibody at (A) 1 and (B) 2 ug/ml.