GAPDH Positive Control siRNA is a synthetic siRNA duplex designed to knock-down human GAPDH mRNA expression. The synthetic siRNAs (5’-GUG GAG AUU GUU GCC AUC AdTdT-3’ and 5’- UGA UGG CAA CAA UCU CCA CdTdT-3’) enable qualitative assessment of knockdown and for optimizing RNAi experiments.
Catalog No. G13-911B
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Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) catalyzes the sixth step of glycolysis – the oxidative phosphorylation of glyceraldehyde-3-phosphate – in the metabolic breakdown of glucose. GAPDH also plays an important role across a diverse array of biological functions such as endocytosis, mRNA regulation, and tRNA export, DNA replication, DNA repair, and apoptosis . GAPDH also serves as an ideal loading control or housekeeping gene .
1. Tarze, A, et al: GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilization. Oncogene. 2007. 26 (18): 2606-2620. PMID 17072346.
2. Said, HM, et al: GAPDH is not regulated in human glioblastoma under hypoxic conditions. BMC Mol Biol. 2007 Jun 27;8:55. PMID 17597534.
GAPDH Positive Control siRNA is designed to specifically knock-down human GAPDH expression.
Single PAGE band
Briefly centrifuge the tubes (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend the siRNA in 25 ul of DEPC-treated water (supplied by researcher), which results in a 1x stock solution (10 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Storage and Stability:
The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.
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