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GAPDH Positive Control siRNA

GAPDH Positive Control siRNA is a synthetic siRNA duplex designed to knock-down human GAPDH mRNA expression. The synthetic siRNAs (5’-GUG GAG AUU GUU GCC AUC AdTdT-3’ and 5’- UGA UGG CAA CAA UCU CCA CdTdT-3’) enable qualitative assessment of knockdown and for optimizing RNAi experiments.
Catalog No. G13-911B


For enquiry on all siRNA Controls email us at orders@signalchem.com


Catalog No. Pack Size Price (USD)
G13-911B-025 2.5 nmol $66
G13-911B-BULK BULK Contact Us  


Overview:

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) catalyzes the sixth step of glycolysis – the oxidative phosphorylation of glyceraldehyde-3-phosphate – in the metabolic breakdown of glucose. GAPDH also plays an important role across a diverse array of biological functions such as endocytosis, mRNA regulation, and tRNA export, DNA replication, DNA repair, and apoptosis [1]. GAPDH also serves as an ideal loading control or housekeeping gene [2].

References:


1. Tarze, A, et al: GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilization. Oncogene. 2007. 26 (18): 2606-2620. PMID 17072346.

2. Said, HM, et al: GAPDH is not regulated in human glioblastoma under hypoxic conditions. BMC Mol Biol. 2007 Jun 27;8:55. PMID 17597534.


Specificity:

GAPDH Positive Control siRNA is designed to specifically knock-down human GAPDH expression.


Purity:

Single PAGE band


Reconstitution Protocol:

Briefly centrifuge the tubes (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend the siRNA in 25 ul of DEPC-treated water (supplied by researcher), which results in a 1x stock solution (10 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.


Format:

Lyophilized powder


Storage and Stability:

The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.



Product Datasheets



RESEARCH AREAS

Apoptosis/Autophagy, Metabolic Disorder


RELATED PRODUCTS


  Anti-GAPDH, G13-61M

  Anti-GAPDH, G13-63R


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