beta-Actin Positive Control siRNA is a synthetic siRNA duplex designed to knock-down human beta-Actin mRNA expression. The synthetic siRNAs (5’-UGA AGA UCA AGA UCA UUG CdTdT-3’ and 5’-GCA AUG AUC UUG AUC UUC AdTdT-3’) enable qualitative assessment of knockdown and for optimizing RNAi experiments.
Catalog No. A03-911D
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Actin, a ubiquitous protein in eukaryotes, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle beta- and gamma-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controling cell structure and motility (1). alpha-cardiac and alpha-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, alpha- and gamma-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate contractile potentials for muscle cells (1).
1. Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48–55.
beta-Actin Positive Control siRNA is designed to specifically knock-down human beta-Actin expression.
Single PAGE band
Briefly centrifuge the tubes (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend the siRNA in 25 ul of DEPC-treated water (supplied by researcher), which results in a 1x stock solution (10 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Storage and Stability:
The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.
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