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beta-Actin Positive Control siRNA

beta-Actin Positive Control siRNA is a synthetic siRNA duplex designed to knock-down human beta-Actin mRNA expression. The synthetic siRNAs (5’-UGA AGA UCA AGA UCA UUG CdTdT-3’ and 5’-GCA AUG AUC UUG AUC UUC AdTdT-3’) enable qualitative assessment of knockdown and for optimizing RNAi experiments.
Catalog No. A03-911D


For enquiry on all siRNA Controls email us at orders@signalchem.com


Catalog No. Pack Size Price (USD)
A03-911D-025 2.5 nmol $66
A03-911D-BULK BULK Contact Us  


Overview:

Actin, a ubiquitous protein in eukaryotes, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle beta- and gamma-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controling cell structure and motility (1). alpha-cardiac and alpha-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, alpha- and gamma-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate contractile potentials for muscle cells (1).

References:


1. Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48–55.


Specificity:

beta-Actin Positive Control siRNA is designed to specifically knock-down human beta-Actin expression.


Purity:

Single PAGE band


Reconstitution Protocol:

Briefly centrifuge the tubes (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend the siRNA in 25 ul of DEPC-treated water (supplied by researcher), which results in a 1x stock solution (10 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.


Format:

Lyophilized powder


Storage and Stability:

The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.



Product Datasheets



RESEARCH AREAS

Cancer, Invasion/Metastasis


RELATED PRODUCTS


  Anti-beta-Actin, A03-63BR

  Anti-beta-Actin, A03-60BM


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