Full length recombinant human CK1 gamma 2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. C68-10BG
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
C68-10BG-05 | 5 ug | $226 | |
C68-10BG-10 | 10 ug | $325 | |
C68-10BG-BULK | BULK | Contact Us |
Overview:
CK1γ2 is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair. CK1γ2 is a ubiquitously expressed cytoplasmic kinase that can interact and phosphorylate the metastatic tumor antigen 1 short form (MTA1s) co-localizing it in the cytoplasm (1). Phosphorylated MTA1s then sequesters the estrogen receptor-alpha in the cytoplasm of breast cancer cells. CK1γ2 hyperphosphorylates the ceramide transfer protein CERT leading to a decrease in de novo sphingomyelin synthesis. The reduction in synthesis of sphingomyelin is reversed by the expression of CERT mutants that are not hyperphosphorylated (2).
Gene Aliases:
CSNK1G2, CK1G2
Genbank Number:
References:
1. Mishra, S K. et al: Metastatic tumor antigen 1 short form (MTA1s) associates with casein kinase I-gamma2, an estrogen-responsive kinase. Oncogene. 2004 May 27;23(25):4422-9.
2. Tomishige N. et al: Casein kinase I{gamma}2 down-regulates trafficking of ceramide in the synthesis of sphingomyelin. Mol Biol Cell. 2009 Jan;20(1):348-57.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~73 kDa
R Chia et al., Phosphorylation of LRRK2 by casein kinase 1α regulates trans-Golgi clustering via differential interaction with ARHGEF7. Nature Communications December 2014 10.1038/ncomms6827
A. Basta Holly et al., Encephalomyocarditis Virus Leader Is Phosphorylated by CK2 and Syk as a Requirement for Subsequent Phosphorylation of Cellular Nucleoporins Journal of Virology December 2013 10.1128/JVI.03150-13
Mamais Adamantios et al., Arsenite Stress Down-regulates Phosphorylation and 14-3-3 Binding of Leucine-rich Repeat Kinase 2 (LRRK2), Promoting Self-association and Cellular Redistribution Journal of Biological Chemistry June 2014 10.1074/jbc.M113.528463
Cancer, Inflammation, Neurobiology, NfkB Pathway, Ser/Thr Kinases, WNT Signaling
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