ULK1, Active
Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. U01-11G
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| U01-11G-05 | 5 ug | $259 | |
| U01-11G-10 | 10 ug | $380 | |
| U01-11G-BULK | BULK | Contact Us |
Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. U01-11G
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| U01-11G-05 | 5 ug | $259 | |
| U01-11G-10 | 10 ug | $380 | |
| U01-11G-BULK | BULK | Contact Us |
Overview:
ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR (1). ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy (2). Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
Gene Aliases:
ATG1, UNC51, Unc51.1, FLJ38455
Genbank Number:
References:
1. Ganley I G, et al: ULK1.ATG13.FIP200 complex mediates mTOR signaling and is essential for autophagy. J Biol Chem. 2009 May 1;284(18):12297-305.
2. Chan E Y, et al: siRNA screening of the kinome identifies ULK1 as a multidomain modulator of autophagy. J Biol Chem. 2007 Aug 31;282(35):25464-74.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
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Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
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Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~125 kDa
Antonioli M et al., Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains
Science Advances May 2019 10.1126/sciadv.aau8857
Guha P et al., IPMK Mediates Activation of ULK Signaling and Transcriptional Regulation of Autophagy Linked to Liver Inflammation and Regeneration
Cell Reports March 2019 10.1016/j.celrep.2019.02.013
Saleiro D et al., IFN-γ-inducible antiviral responses require ULK1-mediated activation of MLK3 and ERK5 Science Signaling November 2018 10.1126/scisignal.aap9921
Shen J et al., Conservation of structure, function and inhibitor binding in UNC-51-like kinase 1 and 2 (ULK1/2)
Biochemical Journal August 2019 10.1042/BCJ20190038
JM Park et al., The ULK1 complex mediates MTORC1 signaling to the autophagy initiation machinery via binding and phosphorylating ATG14. Autophagy April 2016 10.1080/15548627.2016.1140293
et al., TBK1 at the crossroads of inflammation and energy homeostasis in adipose tissue Cell February 2019
et al., ULK1 Phosphorylates and Regulates Mineralocorticoid Receptor. Cell reports December 2019
et al., ULK1 phosphorylates Ser30 of BECN1 in association with ATG14 to stimulate autophagy induction Autophagy February 2018
et al., Histidine dephosphorylation of the G? protein GPB ?1 promotes axon regeneration in C.?elegans EMBO Reports October 2022
et al., Phosphorylation of the LIR Domain of SCOC Modulates ATG8 Binding Affinity and Specificity Journal of Molecular Biology June 2021
Apoptosis/Autophagy, Cancer, Ser/Thr Kinases
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