Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
R15-63BR-100	100 ug	$270
R15-63BR-BULK	BULK	Contact Us

Overview:

The RSK or 90-kDa ribosomal S6 kinase family of proteins is a group of highly conserved Ser/Thr kinases that regulate diverse cellular processes during the growth factor mediated stimulation of cell proliferation, survival and differentiation (1). In mammals, four RSK genes have been identified (RSK1, RSK2, RSK3 and RSK4), and the RSK isoforms are composed of two distinct and functional kinase domains that are activated in a sequential manner by a series of phosphorylation events. RSKs are downstream effectors of the Ras-extracellular signal regulated kinase (ERK) / mitogen-activated protein kinase (MAPK) signalling cascade.

References:

1. Anjum R. et al:The RSK family of kinases: emerging roles in cellular signalling.Nat Rev Mol Cell Biol. 2008 Oct;9(10):747- 58.

Specificity:

Recognizes the RSK1, RSK2, and RSK3 proteins

Cross Reactivity:

Western blot RSK1, RSK2, and RSK3 from human cells

RSK1, RSK2, and RSK3 from other species may also be detectable.

Host :

Rabbit

Immunogen:

Synthetic peptide corresponding to amino acids 346-350 of mouse p90 RSK (protein accession number P18653)

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Representative western blot with Anti-RSK (pan) (1:1000) using 5 ng of human recombinant RSK1, RSK2, and RSK3.