Rabbit Polyclonal Antibody
Catalog No. E10-65ER
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
E10-65ER-100 | 100 ug | $270 | |
E10-65ER-BULK | BULK | Contact Us |
Overview:
The epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase. Known ligands of EGFR include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding induces receptor homo- and hetero-dimerization and tyrosine autophosphorylation, which triggers downstream signaling events. The consequences of EFGR signaling include cell proliferation, differentiation, motility, and cell survival (1). Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa and EGFR upregulated in colon cancers and most neoplasms (2). EGFR stimulation also activates the ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. In contrast, selective inhibition of EGFR significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation. Mutations in EGFR are also implicated in specific forms of lung cancer.
References:
1. Wang K, et al: Epidermal growth factor receptor-deficient mice have delayed primary endochondral ossification because of defective osteoclast recruitment. J. Biol. Chem. 279: 53848-53856, 2004.
2. Kobayashi S, et al: EGFR mutation and resistance of non-small-cell lung cancer to gefitinib. New Eng. J. Med. 352: 786-792, 2005.
Specificity:
Recognizes the EGFR protein phosphorylated at tyrosine 1110
Cross Reactivity:
Human, Mouse and Rat
Host / Isotype / Clone#:
Rabbit, IgG
Immunogen:
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1110
Purification:
Affinity chromatography
Stability:
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Western blot analysis of extracts from A431 cells untreated or treated with EGF (200ng/ml, 5min), using EGFR antibody (lanes 1 and 2) and anti-phospho-EGFR (Tyr1110) antibody (lanes 3 and 4).
DE Heppner et al., The NADPH Oxidases DUOX1 and NOX2 Play Distinct Roles in Redox Regulation of Epidermal Growth Factor Receptor Signaling. Journal of Biological Chemistry October 2016 10.1074/jbc.M116.749028
Krejci Pavel et al., NF449 Is a Novel Inhibitor of Fibroblast Growth Factor Receptor 3 (FGFR3) Signaling Active in Chondrocytes and Multiple Myeloma Cells Journal of Biological Chemistry July 2010 10.1074/jbc.M109.083626
Lia Feng et al., Screening of epidermal growth factor receptor inhibitors in natural products by capillary electrophoresis combined with high performance liquid chromatography-tandem mass spectrometry Journal of Chromatography A June 2015 10.1016/j.chroma.2015.04.055
Rao Suman et al., Target Modulation by a Kinase Inhibitor Engineered to Induce a Tandem Blockade of the Epidermal Growth Factor Receptor (EGFR) and c-Src: The Concept of Type III Combi-Targeting PLoS One February 2015 10.1371/journal.pone.0117215
Krejci Pavel et al., Receptor Tyrosine Kinases Activate Canonical WNT/β-Catenin Signaling via MAP Kinase/LRP6 Pathway and Direct β-Catenin Phosphorylation PLoS One April 2012 10.1371/journal.pone.0035826
AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Metabolic Disorder, PKA/PKC Pathway, Receptor Tyrosine Kinases
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