Recombinant full-length human CDK9 and CyclinT2 proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. C40-18BG
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CDK9/Cyclin T2 is a member of the cyclin-dependent protein kinase (CDK) family which are known to be important regulators of cell cycle progression. Cyclin T2 and its kinase partner CDK9 are found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin has been reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein (1). The transcription-dependent interaction of P-TEFb with 7SK may therefore contribute to an important feedback loop modulating the activity of RNA Pol II (2).
CDK9: CDC2L4, C-2k, TAK, PITALRE
CyclinT2: CCNT2; CYCT2
1. Peng, J.et.al: Identification of multiple cyclin subunits of human P-TEFb. Genes Dev. 12: 755-762, 1998.
2. Nguyen, V. T.et.al: 7SK small nuclear RNA binds to and inhibits the activity of CDK9/cyclin T complexes.Nature 414: 322-325, 2001.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
~68 kDa/105 kDa
M Kanke et al., Cohesin acetylation and Wapl-Pds5 oppositely regulate translocation of cohesin along DNA. EMBO Journal November 2016 10.15252/embj.201695756
Shiozaki Y et al., The CDK 9–cyclin T1 complex mediates saturated fatty acid–induced vascular calcification by inducing expression of the transcription factor CHOP The journal of Biological Chemistry September 2018 10.1074/jbc.RA118.004706
Cancer, Cell Cycle, Ser/Thr Kinases