Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
I25-63R-100	100 ug	$595
I25-63R-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

I25-63R-100

100 ug

$595

I25-63R-BULK

BULK

Overview:

IKKgamma is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex. IKKgamma forms dimers and trimers and interacts preferentially with IKKbeta but not IKKalpha (1). IKKgamma associates with activated ATM after the induction of DNA double-strand breaks (2). ATM phosphorylates serine-85 of IKKgamma to promote its ubiquitin-dependent nuclear export.

References:

1. Rothwarf, D. M. et al: IKK-gamma is an essential regulatory subunit of the I-kappa-B kinase complex. Nature 395: 297-300, 1998.

2. Wu, Z-H. et al: Molecular linkage between the kinase ATM and NF-kappaB signaling in response to genotoxic stimuli. Science 311: 1141-1146, 2006.

Specificity:

Recognizes the IKK gamma protein and has no cross response to IKK alpha or IKK beta

Cross Reactivity:

Human, Mouse and Rat

Host / Isotype / Clone#:

Rabbit, IgG

Immunogen:

IKK gamma antibody was raised against a peptide corresponding to amino acids 400 to 416 of human IKK gamma, which are identical to those of mouse homologue.

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Western blot analysis of IKK gamma in HeLa whole cell lysate with IKK gamma antibody at 1 ug/ml.