Recombinant Human CXCL12 (SDF-1 alpha) was expressed in E. coli cells.
Catalog No. S821-402N
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| S821-402N-10 | 10 ug | $195 | |
| S821-402N-1000 | 1 mg | $3650 | |
| S821-402N-BULK | BULK | Contact Us |
Overview:
SDF-1-alpha and SDF-1-beta are small cytokines belonging to the CXC-Chemokines. SDF-1 is identical with a chemokine reported to function as a pre-B-cell growth factor in the presence of IL-7 and isolated originally from a murine bone marrow stromal cell line. Human SDF-1-alpha and SDF-1-beta are encoded by a single gene and arise by alternative splicing. SDF-1 acts on lymphocytes and monocytes but not neutrophils in vitro and is a highly potent chemoattractant for mononuclear cells in vivo. In addition. SDF-1 also induces intracellular actin polymerization in lymphocytes. SDF acts as a chemoattractant for human hematopoietic progenitor cells expressing CD34 giving rise to mixed types of progenitors, and more primitive types. The chemotactic response is inhibited by pertussis toxin. Chemotaxis of CD34(+) cells in response to SDF is increased by IL-3 in vitro. SDF has been shown also to induce a transient elevation of cytoplasmic calcium in these cells.
Gene Aliases:
Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, IRH, hIRH, Pre-B cell growth-stimulating factor, PBSF, Recombinant Human Stromal Cell-Derived Factor-1 Alpha (CXCL12).
Genbank Number:
Storage, Stability and Shipping:
The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Endotoxin Level:
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Molecular Weight:
8.0 kDa
Reconstitution Protocol:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Sample Cell Assay Data:
Determined by its ability to chemoattract human U937 expressing CXCR4 Determined by calcium flux with human U937 cells.
Purity:
Recombinant Human CXCL12 (SDF-1 alpha) resolved on a 17% SDS-PAGE gel under non-reducing conditions and stained with Coomassie Brilliant Blue G-250.
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