Storage, Stability and Shipping:

The lyophilized protein is stable for at least one year from date of receipt at -70^oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2^o - 8^oC for one month, or at -20^oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Endotoxin Level:

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Molecular Weight:

8.9 kDa

Reconstitution Protocol:

Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Sample Cell Assay Data:

Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CX3CR1. The ED(50) for this effect is typically 20­100 ng/mL

Purity:

Recombinant Human Fractalkine(CX3CL1) resolved on a 17% SDS_PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250.