Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
G805-40N-10	10 ug	$215
G805-40N-50	50 ug	$600
G805-40N-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

G805-40N-10

10 ug

$215

G805-40N-50

50 ug

$600

G805-40N-BULK

BULK

Overview:

G-CSF is secreted by monocytes, macrophages, and neutrophils after cell activation. It is also produced by stromal cells, fibroblasts, endothelial cells, epithelial carcinomas, acute myeloid leukemia cells, and various tumor cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, IL1 and GM-CSF. Comparison of the primary sequence of G-CSF with those of GM-CSF and M-CSF, shows that the three factors are not related to each other. Murine and human G-CSF share a sequence homology (protein) of approximately 70% and exhibit cross reactivity. G-CSF stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophils and granulocytes lineage in a dose-dependent manner.

Gene Aliases:

GCSF, CSF-3, MGI-1G, Pluripoietin, Human Granulocyte Colony Stimulating Factor (CSF3).

Genbank Number:

P09919

Storage, Stability and Shipping:

The lyophilized protein is stable for at least one year from date of receipt at -70^oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2^o - 8^oC for one month, or at -20^oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Endotoxin Level:

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Molecular Weight:

19.0 kDa

Reconstitution Protocol:

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Sample Cell Assay Data:

The ED50 as determined by the dose-dependent proliferation of neuroblastoma cell line was found to be ≤5ng/ml.