Recombinant Human GDNF was expressed in E. coli cells.
Catalog No. G831-40N
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| G831-40N-10 | 10 ug | $193 | |
| G831-40N-1000 | 1000 ug | $3950 | |
| G831-40N-BULK | BULK | Contact Us |
Overview:
GDNF is a disulfide-linked, homodimeric neurotrophic factor that belongs to the cystine-knot family of growth factors. Natively glycosylated, GDNF signals through a multi-component receptor system that comprises of a RET and one of the four GFRα receptors. In embryonic midbrain cultures, GDNF promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity Recombinant Human GDNF is a non-glycosylated, disulfide-linked homodimer (30 kDa) consisting of 135 amino acids in each subunit.
Gene Aliases:
GDNF, Glial cell line-derived neurotrophic factor, Astrocyte-derived trophic factor, ATF
Recombinant Human Glial-Derived Neurotrophic Factor (GDNF)
Genbank Number:
Storage, Stability and Shipping:
The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Endotoxin Level:
<1.0 EU/μg of recombinant protein as determined by the LAL method
Molecular Weight:
15.0 kDa
Reconstitution Protocol:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Sample Cell Assay Data:
The ED50 as determined by the proliferation of rat C6 cells was found to be <0.1 ng/mL
|
Purity:
Recombinant Human GDNF resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250
|
There are no related publications available for this product.
STAY CONNECTED
Fax: 1-604-232-4601
Toll Free: 1-866-954-6273
info@signalchem.com