Storage, Stability and Shipping:

The lyophilized protein is stable for at least one year from date of receipt at -70^oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2^o - 8^oC for one month, or at -20^oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Endotoxin Level:

<1.0 EU/μg of recombinant protein as determined by the LAL method

Molecular Weight:

16.4 kDa

Reconstitution Protocol:

Reconstitute at 1 mg/mL in sterile 20 mM Tris-­HCI (pH 8.0).

Sample Cell Assay Data:

The ED (50) was determined by the dose-dependent proliferation of human umbilical vein endothelial cells and was found to be <0.5ng/mL.

Purity:

Recombinant Human Leptin (LEP) resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250