Recombinant Human PDGFA was expressed in E. coli cells.
Catalog No. P841-40BN
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| P841-40BN-10 | 10 ug | $595 | |
| P841-40BN-50 | 50 ug | $2100 | |
| P841-40BN-BULK | BULK | Contact Us |
Overview:
The PDGF family is comprised of five different disulphide-linked dimers of four different polypeptide chains: A, B, C and D (PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD). Synthesized mainly by megakaryocytes, PDGFs are stored in the alpha granules of platelets from which they are released following platelet activation. PDGF receptors are expressed in fibroblasts, osteoblasts, chondroblasts, smooth muscle cells, glial cells and endothelial cells. Functioning as an autocrine and paracrine growth factor, PDGFs are involved in a number of biological processes that include but not limited to hyperplasia, chemotaxis, embryonic neuron development, wound healing and respiratory tubule epithelial cell development. Aberrant expression of PDGFs is observed with vascular proliferative diseases such as atherosclerosis. PDGFs regulate the synthesis of their own receptor and also influence the expression of membrane receptors for IL1, EGF, 5-Hydroxytryptamine, LDL and transferrin. Recombinant Human PDGFA is a disulfide-linked homodimer of two A chains.
Gene Aliases:
PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, PDGFA, PDGF-AA, PDGF1, Recombinant Human Platelet-Derived Growth Factor Subunit A (PDGFA)
Genbank Number:
Storage, Stability and Shipping:
The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Endotoxin Level:
<1.0 EU/μg of recombinant protein as determined by the LAL method
Molecular Weight:
14.5 kDa
Reconstitution Protocol:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Sample Cell Assay Data:
The ED50 as determined by the dose-dependent proliferation of mouse embryonic fibroblast cells was found to be ≤ 5.0 ng/mL
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Purity:
Recombinant Human PDGFA resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250
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There are no related publications available for this product.
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