Rabbit Polyclonal Antibody
Catalog No. P05-63R
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p53 is a key regulator of cell growth and acts as a tumor suppressor gene. Wild-type p53 gene can suppress transformation of rat embryo fibroblasts in cell culture by other oncogenes such as the adenovirus E1A and Ras (1). DNA tumor viruses such as SV40 large T antigen and the adenovirus E1A plus E1B-55Kd proteins bind to p53 and inactivate its tumor suppressor activities leading to cellular transformation. Mutational inactivation of the p53 gene is detected in more than 50% of human cancers. Mutation of p53 renders cancer cells more resistant to current cancer therapies due to lack of p53-mediated apoptosis (2).
1. Levine, A J. et al: Tumor suppressor genes: the p53 and retinoblastoma sensitivity genes and gene products. Biochim Biophys Acta. 1990 Jun 1;1032(1):119-36.
2. Sun, Y. p53 and its downstream proteins as molecular targets of cancer. Mol Carcinog. 2006 Jun;45(6):409-15.
Anti-p53 antibody recognizes the p53 protein.
p53 from Human
p53 from other species may also be detectable.
Human p53 peptide (15-40aa)-KLH conjugates
Affinity purified by p53 peptide on agarose from rabbit serum
Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Representative western blot using 5μg, 10μg, and 30μg, of HEK-293 cell lysate.
A. Hilton Benjamin et al., ATR Plays a Direct Antiapoptotic Role at Mitochondria, which Is Regulated by Prolyl Isomerase Pin1 Molecular Cell October 2015 10.1016/j.molcel.2015.08.008
Ling Chau Jenny Fung et al., A crucial role for bone morphogenetic protein-Smad1 signalling in the DNA damage response Nature Communications May 2012 10.1038/ncomms1832
Li Q et al., Genetic analysis of p53 nuclear importation Oncogene December 2007 10.1038/sj.onc.1210597
Graczyk Agnieszka et al., S100A6 Competes with the TAZ2 Domain of p300 for Binding to p53 and Attenuates p53 Acetylation Journal of Molecular Biology September 2013 10.1016/j.jmb.2013.06.007
Chen Dongshi et al., TAp73 promotes cell survival upon genotoxic stress by inhibiting p53 activity Oncotarget September 2014 10.18632/oncotarget.2440
S Mondal et al., A bioluminescent assay for monitoring conjugation of ubiquitin and ubiquitin-like proteins. Analytical Biochemistry October 2016 10.1016/j.ab.2016.06.016
Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Inflammation, JNK/SAPK Pathway, Metabolic Disorder, Neurobiology, p38 Pathway