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Anti-ASK1

Mouse Monoclonal Antibody
Catalog No. M13-60M


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Catalog No. Pack Size Price (USD)
M13-60M-25 25 ug $105
M13-60M-100 100 ug $270
M13-60M-BULK BULK Contact Us  


Overview:

ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6. Overexpression of ASK1 induces apoptotic cell death, and ASK1 is activated in cells treated with tumor necrosis factor-alpha (1). ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation (2).

References:


1. Ichijo, H. et al: Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 275: 90-94, 1997.

2. Nishitoh, H. et al: ASK1 is essential for JNK/SAPK activation by TRAF2. Molec. Cell 2: 389-395, 1998.


Specificity:

Recognizes the ASK1 protein


Cross Reactivity:

Western blot of human ASK1

ASK1 from other species may also be detectable.


Host / Clone#:

Mouse, A1


Immunogen:

Human recombinant 649-946 ASK1


Purification:

Affinity Chromatography


Stability:

Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.


Sample Data:

Representative western blot (1:1000) with Anti-ASK1 (A1) using 20 ng of GST-tagged human full-length recombinant ASK1.



Product Datasheets



There are no related publications available for this product.


RESEARCH AREAS

Apoptosis/Autophagy, Cardiovascular Disease, Cellular Stress, ERK/MAPK Pathway, Ser/Thr Kinases


RELATED PRODUCTS


  ASK1, Active, M13-11G

  Anti-Mouse HRP, G32-62G

  ASK3 Protein, M73-31G


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