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NEK4, Active

Recombinant full-length human NEK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. N06-10G


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Catalog No. Pack Size Price (USD)
N06-10G-05 5 ug $226
N06-10G-10 10 ug $325
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Overview:

NEK4 or Never In Mitosis A-related kinase 4 is a serine/threonine protein kinase. Northern blot show NEK4 is expressed as 2 mRNAs of 4.0 kb and 6.8 kb in heart and several other tissues. NEK4 gene encodes an 841-amino acid polypeptide and the levels of NEK4 mRNA remain invariant throughout the cell cycle (1). Nek4 has been identified in a genetic screen as a mediator of response to Taxol (2). After Taxol treatment, NEK4 promoted microtubule outgrowth, whereas NEK4 deficiency impaired G(2)-M arrest and decreased formation of mitotic-like asters. In contrast, NEK4 deficiency sensitized cells to vincristine, which destabilizes microtubules.


Gene Aliases:

NRK2, STK2


Genbank Number:


References:


1. Levedakou, E. N., et.al: Two novel human serine/threonine kinases with homologies to the cell cycle regulating Xenopus MO15, and NIMA kinases: cloning and characterization of their expression pattern. Oncogene 9: 1977-1988, 1994.

2. Doles, J. et al: Nek4 status differentially alters sensitivity to distinct microtubule poisons. Cancer Res. 2010 Feb 1;70(3):1033-41.


Specific Activity:

Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

~160 kDa



Product Datasheets



There are no related publications available for this product.


RESEARCH AREAS

Cancer, Cell Cycle, Ser/Thr Kinases


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