Recombinant full-length human CAMKK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. C18-10G
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| C18-10G-05 | 5 ug | $226 | |
| C18-10G-10 | 10 ug | $325 | |
| C18-10G-BULK | BULK | Contact Us |
Overview:
CAMKK2 (CAMKKβ) is a member of the CAMKK family. It is broadly distributed in tissues with highest levels in brain, thymus, spleen, and testis (1). CaMKK2 undergoes intramolecular autophosphorylation, is regulated by Ca2+/calmodulin and phosphorylates CaMKI and CaMKIV on Thr177 and Thr200, respectively. CaMKK2 activates both CaMKI and CaMKIV when coexpressed in Jurkat T cells. CaMKK2 also phosphorylates and regulates the activity of AMPK, which is an important regulator of cellular metabolism in response to metabolic stress (2).
Gene Aliases:
CAMKK; CAMKKB; KIAA0787; MGC15254
Genbank Number:
References:
1. Anderson, K.A. et al: Components of a calmodulin-dependent protein kinase cascade. Molecular cloning, functional characterization and cellular localization of Ca2+/calmodulin-dependent protein kinase kinase beta.
2. Hurley, R.L. et al: The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases.
J Biol Chem. 2005 Aug 12;280(32):29060-6.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
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Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
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Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~88 kDa
Q Zhu et al., Adipocyte-specific deletion of Ip6k1 reduces diet-induced obesity by enhancing AMPK-mediated thermogenesis. Journal of Clinical Investigation November 2016 10.1172/JCI85510
Buard Isabelle et al., CaMKII Autonomy Is Required for Initiating But Not for Maintaining Neuronal Long-Term Information Storage Journal Of Neuroscience July 2005 10.1523/JNEUROSCI.1469-10.2010
Wang Ping et al., Beta-arrestin inhibits CAMKKbeta-dependent AMPK activation downstream of protease-activated-receptor-2 BMC Biochemistry September 2010 10.1186/1471-2091-11-36
et al., Nucleus-translocated ACSS2 promotes the gene transcription for lysosomal biogenesis and autophagy Molecular cell June 2018
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