Recombinant full-length human PKAcgamma was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Catalog No. P53-10G
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| P53-10G-05 | 5 ug | $226 | |
| P53-10G-10 | 10 ug | $325 | |
| P53-10G-BULK | BULK | Contact Us |
Overview:
PKA C-gamma (PKAcγ) is a third isoform of the catalytic subunit of cAMP-dependent protein kinase. It was isolated from a human testis cDNA library and was clearly derived from a gene distinct from C-alpha and C-beta and showed tissue-specific expression. Whereas at the amino acid level C-alpha and C-beta showed 93% homology, C-gamma showed only about 80% homology to both C-alpha and C-beta (1). The PRKACG gene is intronless, contains remnants of a poly(A) tail, is flanked by direct repeats, and is co-linear with the PRKACA gene(2).
Gene Aliases:
KAPG; PKAr; cAPKr; PRKACG
Genbank Number:
References:
1. Beebe, S. J. et al: Molecular cloning of a tissue-specific protein kinase (C gamma) from human testis--representing a third isoform for the catalytic subunit of cAMP-dependent protein kinase. Molec. Endocr. 4: 465-475, 1990.
2. Reinton, N. et al: The gene encoding the C gamma catalytic subunit of cAMP-dependent protein kinase is a transcribed retroposon. Genomics 49: 290-297, 1998.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
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Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
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Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~65 kDa
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A Thorne Curtis et al., Small-molecule inhibition of Wnt signaling through activation of casein kinase 1α Nature Chemical Biology November 2010 10.1038/nchembio.453
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S Shah et al., Regulation of FcRγ function by site-specific serine phosphorylation. Journal of Leukocyte Biology September 2016 10.1189/jlb.2AB0516-228R
R Baravalle et al., Impact of R264C and R264H polymorphisms in human aromatase function. Journal of Steroid Biochemistry and Molecular Biology October 2016 10.1016/j.jsbmb.2016.09.022
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Apoptosis/Autophagy, Cardiovascular Disease, ERK/MAPK Pathway, Neurobiology, NfkB Pathway, PKA/PKC Pathway, Ser/Thr Kinases
PKAc beta, Active, P52-10G
AMPK (A1/B1/G2), Active, P55-10H
Modified PKA Substrate, C01-58B
ROCK2, Active, R11M-11H
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