Full-length recombinant human IKKbeta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. I03-18G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
I03-18G-05 | 5 ug | $259 | |
I03-18G-10 | 10 ug | $380 | |
I03-18G-BULK | BULK | Contact Us |
Overview:
IKKbeta is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex. The activity of IKKbeta is stimulated by TNF and IL1 and IKKbeta forms a heterodimer that interacts with NIK (1). Overexpression of catalytically inactive IKKbeta blocks cytokine-induced NF-kappa-B activation. Aspirin and sodium salicylate can specifically inhibit IKKbeta activity in vitro and in vivo by binding to IKKbeta to reduce ATP binding (2).
Gene Aliases:
IKK2, IKBKB, IKKB, NFKBIKB, FLJ40509, IKK-beta, MGC131801
Genbank Number:
References:
1. Woronicz, J D. et al: IkappaB kinase-beta: NF-kappa-B activation and complex formation with IkappaB kinase-alpha and NIK. Science 278: 866-869, 1997.
2. Yin, M.-J. et al: The anti-inflammatory agents aspirin and salicylate inhibit the activity of I-kappa-B kinase-beta. Nature 396: 77-80, 1998.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~115 kDa
Chuang HC et al., GLK-IKKβ signaling induces dimerization and translocation of the AhR-RORγt complex in IL-17A induction and autoimmune disease Science Advances September 2018 10.1126/sciadv.aat5401
AW Sorum et al., Microfluidic Mobility Shift Profiling of Lysine Acetyltransferases Enables Screening and Mechanistic Analysis of Cellular Acetylation Inhibitors. ACS Chemical Biology March 2016 10.1021/acschembio.5b00709
Ha Joh Eun et al., Echinocystic acid ameliorates lung inflammation in mice and alveolar macrophages by inhibiting the binding of LPS to TLR4 in NF-?B and MAPK pathways Biochemical Pharmacology August 2012 10.1016/j.bcp.2012.04.020
Moa Min et al., Phosphorylation of Xenopus p31comet potentiates mitotic checkpoint exit Cell Cycle April 2015 10.1080/15384101.2015.1033590
Ha Joh Eun et al., Kalopanaxsaponin B inhibits LPS-induced inflammation by inhibiting IRAK1 Kinase Cellular Immunology September 2012 10.1016/j.cellimm.2012.10.001
SH Berth et al., HIV Glycoprotein Gp120 Impairs Fast Axonal Transport by Activating Tak1 Signaling Pathways. ASN Neuro November 2016 10.1177/1759091416679073
et al., IKK ? is a ? -catenin kinase that regulates mesenchymal stem cell differentiation JCI Insight January 2018
AKT/PKB Pathway, Apoptosis/Autophagy, Inflammation, p38 Pathway
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