Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
R21-63BR-100	100 ug	$270
R21-63BR-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

R21-63BR-100

100 ug

$270

R21-63BR-BULK

BULK

Overview:

p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6, and is ubiquitously expressed in human adult tissues (1). p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6k activity changes during the cell cycle, and increases 20-fold in G1 cells released from G0 (2). p70S6K activation requires sequential phosphorylation at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as threonine 389, a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK-1).

References:

1. Ferrari, S. et al: S6 phosphorylation and the p70s6k/p85s6k. Crit Rev Biochem Mol Biol. 1994;29(6):385-413. Review.

2. Edelmann, HM. Et al: Cell cycle regulation of p70 S6 kinase and p42/p44 mitogen-activated protein kinases in Swiss mouse 3T3 fibroblasts. J Biol Chem. 1996 Jan 12;271(2):963-71.

Specificity:

Recognizes the p70S6K protein

Cross Reactivity:

Western blot p70S6K from human and mouse cells

p70S6K from other species may also be detectable.

Host :

Rabbit

Immunogen:

Synthetic peptide corresponding to amino acids 409-413 of human p70S6K (protein accession number P23443)

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Representative western blot with Anti-p70S6K (1:1000) using 5 ng of activated His-tag human recombinant p70S6K.