Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
B14-63R-100	100 ug	$595
B14-63R-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

B14-63R-100

100 ug

$595

B14-63R-BULK

BULK

Overview:

BRSK2 is a brain-selective serine/threonine kinase 2 that is mainly expressed in the brain, with weaker expression in testis and pancreas. BRSK2 expressed in insect cells specifically phosphorylates WEE1A, CDC25C and CDC25B in an in vitro assay. DNA damage induced by ultraviolet (UV) irradiation or methyl methane sulfonate, but not by ionizing radiation, enhanced endogenous BRSK2 kinase activity in a caffeine-sensitive manner and caused translocation of BRSK2 from the cytoplasm to the nucleus (1). Overexpression of BRSK2 induces G2/M arrest in HeLa cells while small interfering RNA against BRSK2 partly abrogated UV-induced G2/M arrest. Mammalian BRSK2 kinases are required for neuronal polarization (2).

References:

1. Lu, R. et.al: Human SAD1 kinase is involved in UV-induced DNA damage checkpoint function. J. Biol. Chem. 279: 31164-31170, 2004.

2. Kishi,M.et.al: Mammalian SAD kinases are required for neuronal polarization. Science 307: 929-932, 2005.

Specificity:

Recognizes the BRSK1protein

Cross Reactivity:

Human and Mouse

Host / Isotype / Clone#:

Rabbit, IgG

Immunogen:

BRSK2 antibody was raised against a 23 amino acid synthetic peptide from near the carboxy terminus of human BRSK2.

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Western blot analysis of BRSK2 in human brain tissue lysate with BRSK2 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.