Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies

Catalog No.	Pack Size	Price (USD)
B25-63BR-100	100 ug	$595
B25-63BR-BULK	BULK	Contact Us

Catalog No.

Pack Size

Price (USD)

B25-63BR-100

100 ug

$595

B25-63BR-BULK

BULK

Overview:

BCL2 gene encodes an integral inner mitochondrial membrane protein that acts as an antiapoptotic protein (1). The protein BAD can antagonize both the cell cycle and antiapoptotic functions of BCL2 through binding to the BH3 domain. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma (2). BCL2 is phosphorylated on specific serine/threonine residues within the unstructured loop in response to diverse stimuli and such phosphorylation has been associated with the loss of the biological function of BCL2.

References:

1. Hockenbery, D. et al: Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348: 334-336, 1990.

2. Tsujimoto, Y. et al: Involvement of the bcl-2 gene in human follicular lymphoma. Science 228: 1440-1443, 1985.

Specificity:

Recognizes the BCL2 protein

Cross Reactivity:

Human and Mouse

Host / Isotype / Clone#:

Rabbit, IgG

Immunogen:

BCL2 antibody was raised against a peptide corresponding to 15 amino acids near the N terminus of human BCL2

Purification:

Affinity chromatography

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Western blot analysis of BCL2 in Daudi cell lysates with BCL2 antibody at (A) 1, (B) 2, and (C) 4 ug/ml.