Recombinant full-length human p110 beta and human p85 beta were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Catalog No. P28-102BH
| Catalog No. | Pack Size | Price (USD) | |
|---|---|---|---|
| P28-102BH-05 | 5 ug | $259 | |
| P28-102BH-10 | 10 ug | $380 | |
| P28-102BH-BULK | BULK | Contact Us |
Overview:
PI3K phosphorylate the 3-prime OH position of the inositol ring of inositol lipids which functions in signaling pathways that regulate cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kDa catalytic subunit and an 85-kDa adaptor subunit (1). PI3K plays a critical role in regulating the formation and stability of alpha-2B-beta-3 integrin adhesion bonds, which are necessary for shear force-induced platelet activation. PI3K sustains alpha-2B-beta-3 integrin activation and stabilized platelet aggregation by regulating both integrin-dependent calcium flux and Gi activation of RAP1B (2).
Gene Aliases:
p110beta: PI3K, PIK3C1, PI3Kbeta, MGC133043, p110-BETA
p85beta: PIK3R2, P85B, p85-BETA
References:
1. Hu, P.et.al: Cloning of a novel, ubiquitously expressed human phosphatidylinositol 3-kinase and identification of its binding site on p85. Molec. Cell. Biol. 13: 7677-7688, 1993.
2. ackson, S. P.et.al: PI 3-kinase p110-beta: a new target for antithrombotic therapy. Nature Med. 11: 507-514, 2005.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
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Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
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Storage, Stability, and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
p110beta ~111 kDa and p85beta ~88 kDa
Y Zheng et al., Temporal regulation of EGF signalling networks by the scaffold protein Shc1. Nature July 2013 10.1038/nature12308
FU Rutaganira et al., Design and Structural Characterization of Potent and Selective Inhibitors of Phosphatidylinositol 4 Kinase IIIβ Journal of Medicinal Chemistry March 2016 10.1021/acs.jmedchem.5b01311
ML Lin et al., Suppression of PI3K/Akt signaling by synthetic bichalcone analog TSWU-CD4 induces ER stress-and Bax/Bak-mediated apoptosis of cancer cells Apoptosis : an International Journal on Programmed Cell Death November 2014 10.1007/s10495-014-1031-y
L Maasa Nancy et al., Generation and characterization of an analog-sensitive PERK allele Cancer Biology & Therapy May 2014 10.4161/cbt.29274
Furukawa S et al., PI3Kδ Inhibition Augments the Efficacy of Rapamycin in Suppressing Proliferation of Epstein?Barr Virus (EBV)+ B Cell Lymphomas American Journal of Transplantation July 2013 10.1111/ajt.12328
R Nehme et al., New in-capillary electrophoretic kinase assays to evaluate inhibitors of the PI3k/Akt/mTOR signaling pathway Analytical and Bioanalytical Chemistry May 2014 10.1007/s00216-014-7790-z
Vidugiriene Jolanta et al., Evaluating the Utility of a Bioluminescent ADP-Detecting Assay for Lipid Kinases ASSAY and Drug Development Technologies January 2010 10.1089/adt.2009.0223
et al., Design, Synthesis, and Preclinical Evaluation of Fused Pyrimidine-Based Hydroxamates for the Treatment of Hepatocellular Carcinoma. Journal of medicinal chemistry April 2019
AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Lipid Kinases, Metabolic Disorder, Neurobiology, NfkB Pathway, WNT Signaling
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