NEK3 is a member of the NEK family of protein kinases that share high amino acid homology with NIMA (never in mitosis gene a). NEK3 mRNA is detected in all the proliferating cell lines with the amount not changing during the cell cycle (1). During Prolactin receptor signaling, VAV2 is phosphorylated and activated by NEK3. Overexpression of NEK3 in Chinese hamster ovary cells increases cytoskeletal re-organization in response to Prolactin while downregulation of NEK3 expression by siRNA block these effects (2). Prolactin also stimulates interaction between NEK3 and paxillin leading to increased paxillin phosphorylation, Analysis of breast tissue microarrays show a significant up-regulation of NEK3 expression in malignant versus normal specimens.
1.Kimura, M. et al: Molecular cloning and characterization of the human NIMA-related protein kinase 3 gene (NEK3). Cytogenet Cell Genet. 2001;95(3-4):177-82.
2.Miller, S.L. et al: Nek3 kinase regulates prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells. Oncogene. 2007 Jul 12;26(32):4668-78.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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Cancer, Cell Cycle, Ser/Thr Kinases