Home   >    Enzymes   >   Kinases

CDK6/CyclinD1, Active

Recombinant full-length human CDK6 and CyclinD1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on CDK6 and GST tag on CyclinD1 proteins.
Catalog No. C35-18H


Shop Online



Catalog No. Pack Size Price (USD)
C35-18H-05 5 ug $226
C35-18H-10 10 ug $325
C35-18H-BULK BULK Contact Us  


Overview:

CDK6 is a member of the cyclin-dependent family of protein kinases that are important regulators of cell cycle progression. CDK6 activity is regulated by the D-type cyclins and members of the INK4 family of CDK inhibitors (1). The CDK6 kinase activity is detected in mid-G1 phase of the cell cycle and is responsible for the phosphorylation and regulation of the activity of tumor suppressor protein Rb. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein (2).


Gene Aliases:

CDK6: PLSTIRE, MGC59692 Cyclin D1: BCL1, PRAD1, U21B31, D11S287E


Genbank Number:


References:


1. Meyerson, M. et al: Identification of G1 kinase activity for cdk6, a novel cyclin D partner. Molec. Cell. Biol. 14: 2077-2086, 1994.

2. Takaki, T. et al: Preferences for phosphorylation sites in the retinoblastoma protein of D-type cyclin-dependent kinases, Cdk4 and Cdk6, in vitro. J Biochem. 2005 Mar;137(3):381-6.


Specific Activity:

Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

~40kDa and CyclinD1 ~61kDa



Product Datasheets



Safety Datasheet



RESEARCH AREAS

Cancer, Cell Cycle, Ser/Thr Kinases


RELATED PRODUCTS


  CDKtide, C06-58

  CyclinH Protein, C83-30H

  CDK12/CyclinK Protein, C62-35BG

  CDK13 (CDC2L) Protein, C25-35G


SIGNALCHEM BIOTECH INC.


About

Terms of Service

Privacy Policy

Get the latest resources, updates and offers in your inbox