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PDK1, Active

Recombinant full-length human PDK1 was expressed by baculovirus in Sf9 insect cells using a N-terminal His tag.
Catalog No. P14-10H


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Catalog No. Pack Size Price (USD)
P14-10H-05 5 ug $226
P14-10H-10 10 ug $325
P14-10H-BULK BULK Contact Us  


Overview:

PDK1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns(3,4,5)P3 or PtdIns(3,4)P2 (1). PDK1 then activates protein kinase B (PKB) which, in turn, inactivates glycogen synthase kinase-3 (GSK3). The phosphorylation of other proteins by PKB and GSK3 is likely to mediate many of the intracellular actions of insulin. Thus, PDK1 plays a key role in mediating many of the actions of the second messenger(s) PtdIns(3,4, 5)P3 and/or PtdIns(3,4)P2. The human PDK1 is a 556-residue monomeric enzyme comprising of a catalytic domain that is most similar to the PKA, PKB and PKC subfamily of protein kinases.


Gene Aliases:

PRO0461; PDPK1; MGC20087; MGC35290; PkB-like; PkB-like 1


Genbank Number:


References:


1. Cohen, P. et al: PDK1, one of the missing links in insulin signal transduction? FEBS Letter. 1997 Jun 23;410(1):3-10. Review.

2. Alessi, DR. et al: Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase B alpha. Curr Biol. 1997 Apr 1;7(4):261-9.


Specific Activity:

Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

~67 kDa



Product Datasheets



Safety Datasheet



RESEARCH AREAS

AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, Ser/Thr Kinases, WNT Signaling


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