Recombinant full-length human AMPK (combination of A1/B1/G2 subunits) was expressed by baculovirus in Sf9 insect cells using the N-terminal GST and C-terminal His tags.
Catalog No. P55-10G
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AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis (1). AMPK exists as a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits. Binding of AMP to the gamma subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) (2) and via signaling pathways in response to adiponectin, leptin and CAMKKbeta.
Subunit A1: PRKAA1, MGC33776, MGC57364 Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785 Subunit G2: PRKAG2, AAKG, CMH6, WPWS, AAKG2, H91620p
1. Hardie, G.D. The AMP-activated protein kinase pathway – new players upstream and downstream. J. Cell Sci. 2004;117: 5479–5487.
2. Kahn, B.B. et al. AMP-activated protein kinase: Ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab; 2005: 1, 15–25.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
~92kDa (A1), ~65kDa (B1), and ~105Da (G2)
PM Anders et al., The KSHV K1 Protein Modulates AMPK Function to Enhance Cell Survival. PLoS Pathogens November 2016 10.1371/journal.ppat.1005985
Choi Jiwon et al., Sanguinarine is an allosteric activator of AMP-activated protein kinase Biochemical and Biophysical Research Communications September 2011 10.1016/j.bbrc.2011.08.081
B Eric et al., Discovery of TBC1D1 as an Insulin-, AICAR-, and Contraction-stimulated Signaling Nexus in Mouse Skeletal Muscle Journal of Biological Chemistry April 2008 10.1074/jbc.M708839200
Apoptosis/Autophagy, Cancer, Cellular Stress, Metabolic Disorder, Ser/Thr Kinases