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PFKFB3 (S269A), Active

Recombinant full-length human PFKFB3 (S269A) mutant was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. P323-10BG


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Catalog No. Pack Size Price (USD)
P323-10BG-05 5 ug $259
P323-10BG-10 10 ug $380
P323-10BG-BULK BULK Contact Us  


Overview:

PFKFB3 or 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 is a potent activator of a key regulatory enzyme in the glycolysis pathway called phosphofructokinase. Phosphofructokinase regulates the steady-state concentration of fructose-2,6-bisphosphate, a potent activator of key regulatory enzyme of the glycolysis pathway. PFKFB3 is bifunctional, although the phosphatase activity is low due to a mutation in an active site which can be upregulated after phosphorylation by protein kinases (1). PFKFB3 may be responsible for sustaining the high glycolytic flux of rapidly proliferating leukemia cells and may represent a molecular target for chemotherapeutic intervention (2).


Gene Aliases:

IPFK2; PFK2


Genbank Number:


References:


1. Sakakibara, R.et.al: Characterization of a human placental fructose-6-phosphate,2-kinase/fructose-2,6-bisphosphatase. J. Biochem. 122: 122-128, 1997.

2. Chesney, J. et.al: An inducible gene product for 6-phosphofructo-2-kinase with an AU-rich instability element: role in tumor cell glycolysis and the Warburg effect. Proc. Nat. Acad. Sci. 96: 3047-3052, 1999.


Specific Activity:

Sample Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.


Storage, Stability, and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

85 kDa



Product Datasheets



There are no related publications available for this product.


RESEARCH AREAS

Cancer, Cell Cycle


RELATED PRODUCTS


  Fructose Kinase Assay Buffer I, F01-09

  Fructose Kinase Dilution Buffer I, F21-09

  D-Glucose-6-phosphate, F521-59

  D-Fructose-6-phosphate, F520-59


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