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EGFR, Active

Recombinant human EGFR (668-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. E10-112G


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Catalog No. Pack Size Price (USD)
E10-112G-10 10 ug $325
E10-112G-05 5 ug $226
E10-112G-BULK BULK Contact Us  


Overview:

EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity. Binding of epidermal growth factor to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility, and cell survival. Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa, and its expression is upregulated in colon cancers and most neoplasms. Activation of EGFR triggers activation of the ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation.


Gene Aliases:

ERBB, mENA, ERBB1, HER1


Genbank Number:


References:


1. Wang K, et al: Epidermal growth factor receptor-deficient mice have delayed primary endochondral ossification because of defective osteoclast recruitment. J. Biol. Chem. 279: 53848-53856, 2004.

2. Kobayashi S, et al: EGFR mutation and resistance of non-small-cell lung cancer to gefitinib. New Eng. J. Med. 352: 786-792, 2005.


Storage, Stability, and Shipping:

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.


Molecular Weight:

89~100kDa


Specific Activity:

Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.


Purity:

Sample Purity Data. For specific information on a given lot, see related technical data sheet.



 Ruilong L et al., Tyrosine phosphorylation activates 6-phosphogluconate dehydrogenase and promotes tumor growth and radiation resistance Nature Communications March 2019 10.1038/s41467-019-08921-8

 Martin R et al., Molecular analysis of the dual targeting of the epidermal growth factor receptor and the O6-methylguanine-DNA methyltransferase with a double arm hybrid molecule Oncotarget October 2018 10.18632/oncotarget.25120

 Kobayashi Y et al., Clozapine-dependent inhibition of EGF/neuregulin receptor (ErbB) kinases. Transnational Psychiatry August 2019 10.1038/s41398-019-0519-1

 DE Heppner et al., The NADPH Oxidases DUOX1 and NOX2 Play Distinct Roles in Redox Regulation of Epidermal Growth Factor Receptor Signaling. Journal of Biological Chemistry October 2016 10.1074/jbc.M116.749028

 Krejci Pavel et al., NF449 Is a Novel Inhibitor of Fibroblast Growth Factor Receptor 3 (FGFR3) Signaling Active in Chondrocytes and Multiple Myeloma Cells Journal of Biological Chemistry July 2010 10.1074/jbc.M109.083626

 K Danyal et al., Acrolein and thiol-reactive electrophiles suppress allergen-induced innate airway epithelial responses by inhibition of DUOX1 and EGFR. American Journal of Physiology - Lung Cellular and Molecular Physiology November 2016 10.1152/ajplung.00276.2016

 Lia Feng et al., Screening of epidermal growth factor receptor inhibitors in natural products by capillary electrophoresis combined with high performance liquid chromatography-tandem mass spectrometry Journal of Chromatography A June 2015 10.1016/j.chroma.2015.04.055

 Rao Suman et al., Target Modulation by a Kinase Inhibitor Engineered to Induce a Tandem Blockade of the Epidermal Growth Factor Receptor (EGFR) and c-Src: The Concept of Type III Combi-Targeting PLoS One February 2015 10.1371/journal.pone.0117215

  Krejci Pavel et al., Receptor Tyrosine Kinases Activate Canonical WNT/β-Catenin Signaling via MAP Kinase/LRP6 Pathway and Direct β-Catenin Phosphorylation PLoS One April 2012 10.1371/journal.pone.0035826


RESEARCH AREAS

AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Metabolic Disorder, PKA/PKC Pathway, Receptor Tyrosine Kinases


RELATED PRODUCTS


  EGFR, Active, E10-112H


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