Rabbit Polyclonal Antibody
Catalog No. M13-63R
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
M13-63R-100 | 100 ug | $595 | |
M13-63R-BULK | BULK | Contact Us |
Overview:
ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6. Overexpression of ASK1 induces apoptotic cell death, and ASK1 is activated in cells treated with tumor necrosis factor-alpha (1). ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation (2).
References:
1. Smith, C. Ichijo, H. et al: Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 275: 90-94, 1997.
2. Nishitoh, H. et al: ASK1 is essential for JNK/SAPK activation by TRAF2. Molec. Cell 2: 389-395, 1998.
Specificity:
Recognizes the ASK1 protein
Cross Reactivity:
Human
Host / Isotype / Clone#:
Rabbit, IgG
Immunogen:
ASK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1. This sequence is different from that of mouse by last two amino acids.
Purification:
Affinity chromatography
Stability:
Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data:
Western blot analysis of ASK1 in SW1353 whole cell lysate with ASK1 antibody at 1:500 dilution.
Sample Data:
Immunocytochemistry of ASK1 in A431 cells with ASK1 antibody at 10 ug/ml.
There are no related publications available for this product.
Apoptosis/Autophagy, Cardiovascular Disease, Cellular Stress, ERK/MAPK Pathway, Ser/Thr Kinases
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