Rabbit Polyclonal Antibody
Catalog No. A89-365R
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
A89-365R-100 | 100 ug | $675 | |
A89-365R-BULK | BULK | Contact Us |
Overview:
Cdc27 (also known as cell division cycle protein 27 homologue, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. Cdc27 is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Cdc27 has a nuclear localization.
References:
1. Gabellini,D., et al.: Early mitotic degradation of the homeoprotein HOXC10 is potentially linked to cell cycle progression. EMBO J. 2003: 22 (14); 3715-3724.
2. Topper,L.M., et al. The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis. Cell Cycle. 2002:1 (4); 282-292.
3. Wassmann,K., et al.: Mad2 transiently associates with an APC/p55Cdc complex during mitosis. Proc. Natl. Acad. Sci. U.S.A. 1988: 95 (19); 11193-11198.
Specificity:
Recognizes the Cdc27 protein phosphorylated at Threonine 244
Cross Reactivity:
Human
Host Isotype / Clone#:
Rabbit, IgG
Immunogen:
The antibody was produced against synthesized peptide corresponding to amino acids 239-240 of Human Cdc27 protein.
Purification:
Immunoaffinity chromatography
Stability:
1yr at –20oC from date of shipment
Sample Data:
Western blot using Affinity Purified anti-Phospho-APC3 (Thr244) (1:500) to detect phosphorylated human CDC27 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. Each lane contains approximately 30µg of HeLa whole cell lysates.
There are no related publications available for this product.
Cancer, Cell Cycle, Neurobiology, p38 Pathway
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