Rabbit Polyclonal Antibody
Catalog No. M13-65BR
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
M13-65BR-200 | 200 ug | $675 | |
M13-65BR-BULK | BULK | Contact Us |
Overview:
ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6. Overexpression of ASK1 induces apoptotic cell death, and ASK1 is activated in cells treated with tumor necrosis factor-alpha (1). ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation (2).
References:
1. Ichijo, H. et al: Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 275: 90-94, 1997.
2. Nishitoh, H. et al: ASK1 is essential for JNK/SAPK activation by TRAF2. Molec. Cell 2: 389-395, 1998.
Specificity:
Recognizes the ASK1 protein phosphorylated at Serine 83
Cross Reactivity:
Human
Host Isotype / Clone#:
Rabbit, IgG
Immunogen:
The antibody was produced against synthesized peptide corresponding to amino acids 76-87 on human ASK1 protein.
Purification:
Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
Stability:
1yr at –20oC from date of shipment
Sample Data:
Western blot of Anti-Phospho-ASK1 (Ser83) against Cos-7 cell lysates after transient infection with 1) vector only, 2) recombinant HA-ASK1, and 3) recombinant human HA-ASK1 (Ser83 substituted with Ala residue).
There are no related publications available for this product.
Apoptosis/Autophagy, Cancer, Cellular Stress, JNK/SAPK Pathway, p38 Pathway, Ser/Thr Kinases
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