Rabbit Polyclonal Antibody
Catalog No. M52-65R
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
M52-65R-100 | 100 ug | $675 | |
M52-65R-BULK | BULK | Contact Us |
Overview:
c-MET is a receptor tyrosine kinase that binds hepatocyte growth factor (also known as scatter factor, HGF/SF). Stimulation by HGF causes c-MET to autophosphorylate at multiple tyrosines, including Y1234 and Y1235. Activated c-MET binds to various substrates, such as GAB1, GRB2, phosphatidylinositol 3-kinase (PI3K) and others. GAB1 is scaffold protein, which enables c-MET to elicit downstream signaling cascades, which include the well-characterized ERK/MAPK, PI3K–Akt/PKB, Crk–Rap and Rac–Pak pathways; leading to cell proliferation and cell survival.
References:
1. Birchmeier, C., et al.: Met, metastasis, motility and more. Nat. Rev. Mol. Cell Bio. 2003: 4; 915-925.
2. Fan, S., et al.: The multisubstrate adapter Gab1 regulates hepatocyte growth factor (scatter factor)-c-Met signaling for cell survival and DNA repair. Mol. Cell Biol. 2001: 21; 4968-84.
3. Longati, P., et al. Receptor tyrosine kinases as therapeutic targets: the model of the MET oncogene. Curr. Drug Targets. 2001: 2; 41-55.
Specificity :
Recognizes the human c-MET phosphorylated at Tyrosine 1349 and 1356
Cross Reactivity:
Human, Mouse and Rat
Host Isotype / Clone#:
Rabbit, IgG
Immunogen:
The antibody was produced against synthesized peptide corresponding to residues surrounding Tyr1349 and Tyr1356 of human c-MET protein.
Purification:
Immunoaffinity chromatography
Stability:
1yr at –20oC from date of shipment.
Sample Data:
Western Blot using Anti-phospho-MET (Tyr1349 Tyr1356) antibody shows detection of phosphorylated c-Met in Human mammary B5/589 epithelial cells serum-deprived and treated with or without HGF.
Cancer, Receptor Tyrosine Kinases
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